Attachment 10


               FACTSHEET HUMAN EXPERIMENTATION - 189

Project Name:  The Study of Irradiation Effects on the Human
Testis, including Histologic, Chromosomal and Hormonal Aspects

Date Started:  8/63
Date Terminated:    12/70

Institution:   Battelle Pacific Northwest Laboratory
Funding Source(s):  AEC

Identification:     Contract AT(45-1)225, Task Agreement #6
Project Duration:
Principal Investigator(s):    C. Alvin Paulsen, M.D.

Responsible Government Official(s):     Charles L. Dunham, M.D.
                              John R. Totter, Ph.D.

Objective(s) of Project: 1)   To relate radiation dosage to
changes in gonadal function, 2) to utilize the radiation gonadal
changes as means for studying pituitary-testis
interrelationships, 3) to explore therapeutic and medical
protective measures with respect to gonadal irradiation, 4) to
explore our current concepts concerning radiation dosage
expressed in physical terms and ILLEGIBLE relation to biologic
effects.

Short Description:  After appropriate baseline studies were
performed, subjects received from 7.5r to 400r x-ray irradiation
or sham irradiation to the testis only.  Testicular biopsies were
performed prior to an up to 6 years post-irradiation to assess
changes in the germinal epithelium.  Seminal fluid specimens were
obtained at two-week intervals throughout the study and were
analyzed for morphologic changes and changes in total number of
sperm.  Urine specimens were obtained monthly for evaluation of
hormonal changes.  Later, when the techniques were available,
serum LH was measured by radioimmunoassay.  Studies of
ultrastructure changes in testis tissue were performed using
electron microscopy.  Subjects were followed until hormonal
values returned to normal levels and until sperm counts returned
to normal ranges.  Vasectomies were performed prior to discharge
from the study to eliminate the possibility of defective
offspring.  Only individuals desiring vasectomy were accepted for
the study; but in several instances the volunteers changed their
mind and did not desire a vasectomy at the conclusion of the
study.  Subjects were informed in detail regarding the study and
signed consent forms were obtained from subjects and from spouses
if subjects were married.  Subjects were prison inmates only,
thus eliminating the possibility of conception during the study.

Follow-up Data:     (See covering letter)

FACTSH..189 (cont'd)

References:    Attached is the publication list from our final
technical report, dated January 31, 1973.

Attachment(s): Covering letter; reference list.